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whole human genome 4 * 44 k microarrays (g4112f) applying for gene expression profiling  (Agilent technologies)


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    Agilent technologies whole human genome 4 * 44 k microarrays (g4112f) applying for gene expression profiling
    Whole Human Genome 4 * 44 K Microarrays (G4112f) Applying For Gene Expression Profiling, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+gene+expression+profile+microarray/pm36077272-320-7-32
    Average 90 stars, based on 1 article reviews
    whole human genome 4 * 44 k microarrays (g4112f) applying for gene expression profiling - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Gene Expression:

    Article Title: Primary endothelial cell–specific regulation of hypoxia-inducible factor (HIF)-1 and HIF-2 and their target gene expression profiles during hypoxia
    Article Snippet: The Agilent Technologies human transcriptome array (SurePrint G3 Gene Expression Microarray v.3 G4851C) and dedicated Agilent Technologies reagent set were used to analyze the mRNA expression pattern of primary HUVECs. .. The Agilent Technologies human transcriptome array (SurePrint G3 Gene Expression Microarray v.3 G4851C) and dedicated Agilent Technologies reagent set were used to analyze the mRNA expression pattern of primary HUVECs. ..

    Article Title: Transcriptome Profiling of Caco-2 Cancer Cell Line following Treatment with Extracts from Iodine-Biofortified Lettuce ( Lactuca sativa L.)
    Article Snippet: .. The analysis of gene-expression profile was performed using SurePrint G3 Human Gene Expression 8x60K v2 Microarray (Agilent Technologies, Santa Clara, CA, USA). ..

    Microarray:

    Article Title: Primary endothelial cell–specific regulation of hypoxia-inducible factor (HIF)-1 and HIF-2 and their target gene expression profiles during hypoxia
    Article Snippet: The Agilent Technologies human transcriptome array (SurePrint G3 Gene Expression Microarray v.3 G4851C) and dedicated Agilent Technologies reagent set were used to analyze the mRNA expression pattern of primary HUVECs. .. The Agilent Technologies human transcriptome array (SurePrint G3 Gene Expression Microarray v.3 G4851C) and dedicated Agilent Technologies reagent set were used to analyze the mRNA expression pattern of primary HUVECs. ..

    Article Title: Identification of cancer biomarkers of prognostic value using specific gene regulatory networks (GRN): a novel role of RAD51AP1 for ovarian and lung cancers
    Article Snippet: The extracted RNA was quality verified on a 2100 expert Agilent bioanalyzer, all samples had an RNA yield greater than 30 μg and RNA integrity number values of 10 ( , available at Carcinogenesis Online). .. Samples were amplified and labelled with Agilent Low Input Quick Amp labelling two colour kit and hybridized with a dye swap to an Agilent G3 8plex × 60k gene Human transcriptome microarray and washed to supplier’s protocol. .. The array slide was scanned at 3 μM and 20 bit tagged image file format (TIFF) file dynamic range on an Agilent Surescan G2565CA microarray scanner at 100% photomultiplier tube gain for both red and green lasers and exported TIFF images.

    Article Title: Transcriptome Profiling of Caco-2 Cancer Cell Line following Treatment with Extracts from Iodine-Biofortified Lettuce ( Lactuca sativa L.)
    Article Snippet: .. The analysis of gene-expression profile was performed using SurePrint G3 Human Gene Expression 8x60K v2 Microarray (Agilent Technologies, Santa Clara, CA, USA). ..

    Article Title: Identification of cancer biomarkers of prognostic value using specific gene regulatory networks (GRN): a novel role of RAD51AP1 for ovarian and lung cancers.
    Article Snippet: The extracted RNA was quality verified on a 2100 expert Agilent D ow nloaded from https://academ ic.oup.com /carcin/article/39/3/407/4604869 by guest on 19 M arch 2024 bioanalyzer, all samples had an RNA yield greater than 30 μg and RNA integrity number values of 10 (Supplementary Figure 1A, available at Carcinogenesis Online). .. Samples were amplified and labelled with Agilent Low Input Quick Amp labelling two colour kit and hybridized with a dye swap to an Agilent G3 8plex × 60k gene Human transcriptome microarray and washed to supplier’s protocol. .. The array slide was scanned at 3 μM and 20 bit tagged image file format (TIFF) file dynamic range on an Agilent Surescan G2565CA microarray scanner at 100% photomultiplier tube gain for both red and green lasers and exported TIFF images.

    Expressing:

    Article Title: Primary endothelial cell–specific regulation of hypoxia-inducible factor (HIF)-1 and HIF-2 and their target gene expression profiles during hypoxia
    Article Snippet: The Agilent Technologies human transcriptome array (SurePrint G3 Gene Expression Microarray v.3 G4851C) and dedicated Agilent Technologies reagent set were used to analyze the mRNA expression pattern of primary HUVECs. .. The Agilent Technologies human transcriptome array (SurePrint G3 Gene Expression Microarray v.3 G4851C) and dedicated Agilent Technologies reagent set were used to analyze the mRNA expression pattern of primary HUVECs. ..

    other:

    Article Title: Comprehensive analysis reveals novel gene signature in head and neck squamous cell carcinoma: predicting is associated with poor prognosis in patients
    Article Snippet: GSE53819 is a gene expression profile of HNSC and belongs to the Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version), which can be downloaded from the GEO database and executed on the GPL6480 platform ( ).

    Article Title: 5th World Congress of the International Academy of Oral Oncology (IAOO).
    Article Snippet: HEAD & NECK JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK JULY 2015 VOLUME 37/NUMBER S1 CONTENTS E1 Welcome letter E2 Committees E7 Keynote lectures E11 Oral presentations E134 Poster presentations E1 Dear colleagues, It is an honor for us to welcome you all to the 5th World Congress of the International Academy of Oral Oncology in 2015.. On 2015 the International Academy of Oral Oncology is celebrating its 10th anniversary.. IAOO has been drawing together clinicians and scientists worldwide, working on issues relating to the etiopathogenesis, epidemiology, prevention and management of oral and perioral tumors as well as oral diseases in patients with malignancies.

    Article Title: Primary endothelial cell–specific regulation of hypoxia-inducible factor (HIF)-1 and HIF-2 and their target gene expression profiles during hypoxia
    Article Snippet: Gene expression microarray SurePrint G3 processing and hybridization The Agilent Technologies human transcriptome array (SurePrint G3 Gene Expression Microarray v.3 G4851C) and dedicated Agilent Technologies reagent set were used to analyze the mRNA expression pattern of primary HUVECs.

    Article Title: Systems Analysis of the Human Pulmonary Arterial Hypertension Lung Transcriptome
    Article Snippet: Supplemental Figure E3: The PAH classifier was used to supervise an independent PAH lung transcriptome microarray experiment (GSE15197 (8)) which used independently collected and process human lung tissue analyzed on a different microarray platform (Agilent).

    Amplification:

    Article Title: Identification of cancer biomarkers of prognostic value using specific gene regulatory networks (GRN): a novel role of RAD51AP1 for ovarian and lung cancers
    Article Snippet: The extracted RNA was quality verified on a 2100 expert Agilent bioanalyzer, all samples had an RNA yield greater than 30 μg and RNA integrity number values of 10 ( , available at Carcinogenesis Online). .. Samples were amplified and labelled with Agilent Low Input Quick Amp labelling two colour kit and hybridized with a dye swap to an Agilent G3 8plex × 60k gene Human transcriptome microarray and washed to supplier’s protocol. .. The array slide was scanned at 3 μM and 20 bit tagged image file format (TIFF) file dynamic range on an Agilent Surescan G2565CA microarray scanner at 100% photomultiplier tube gain for both red and green lasers and exported TIFF images.

    Article Title: Identification of cancer biomarkers of prognostic value using specific gene regulatory networks (GRN): a novel role of RAD51AP1 for ovarian and lung cancers.
    Article Snippet: The extracted RNA was quality verified on a 2100 expert Agilent D ow nloaded from https://academ ic.oup.com /carcin/article/39/3/407/4604869 by guest on 19 M arch 2024 bioanalyzer, all samples had an RNA yield greater than 30 μg and RNA integrity number values of 10 (Supplementary Figure 1A, available at Carcinogenesis Online). .. Samples were amplified and labelled with Agilent Low Input Quick Amp labelling two colour kit and hybridized with a dye swap to an Agilent G3 8plex × 60k gene Human transcriptome microarray and washed to supplier’s protocol. .. The array slide was scanned at 3 μM and 20 bit tagged image file format (TIFF) file dynamic range on an Agilent Surescan G2565CA microarray scanner at 100% photomultiplier tube gain for both red and green lasers and exported TIFF images.



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    Validation of genes identified using microarray. MCF7 and TAMR cells were transfected with silencer negative control siRNA, ATF2 siRNA1 and ATF2 siRNA2. qRT-PCR was carried out using Taqman primers for the genes indicated. Data analysis for each cell line was carried out relative to the siControl ( n = 3) and asterisks indicate the genes that were significantly changed after ATF2 knockdown (* p < 0.05, ** p < 0.005)

    Journal: Breast Cancer Research : BCR

    Article Title: Activating transcription factor-2 (ATF2) is a key determinant of resistance to endocrine treatment in an in vitro model of breast cancer

    doi: 10.1186/s13058-020-01359-7

    Figure Lengend Snippet: Validation of genes identified using microarray. MCF7 and TAMR cells were transfected with silencer negative control siRNA, ATF2 siRNA1 and ATF2 siRNA2. qRT-PCR was carried out using Taqman primers for the genes indicated. Data analysis for each cell line was carried out relative to the siControl ( n = 3) and asterisks indicate the genes that were significantly changed after ATF2 knockdown (* p < 0.05, ** p < 0.005)

    Article Snippet: We also performed a microarray gene expression profiling (Illumina Human HT12_v4) to explore alterations in gene expression between MCF7 and TAMRs after ATF2 silencing and confirmed gene expression changes by quantitative RT-PCR.

    Techniques: Biomarker Discovery, Microarray, Transfection, Negative Control, Quantitative RT-PCR, Knockdown